Part:BBa_K4047021:Experience

This terminator was able to successfully allow transcription and expression of our selective antibiotic marker kanamycin, allowing transformed colonies to grow on a kanamycin-containing agar plate. This antibiotic resistance marker, with its corresponding promoter and terminator, was used in the plasmid BBa_K4047039. The insertion of the part both disrupted functional gene expression of our target gene and served as a selective marker for successful integration. This part was successfully used in the 2021 MiamiU_OH team project, with a picture shown below of the resulting growth as experimental evidence.

Growth of Synechococcus elongatus PCC 7942 on kanamycin-containing plate after transformation with plasmids containing the kanamycin resistance part BBa_K4047019

Applications of BBa_K4047021

This part can be used in plasmids to allow functional transcription and expression of the selective marker kanamycin, indicating when colonies have been successfully transformed.

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